The crystal structure of 2,4-diamino-6-nitro-5-(p-tolyl)-7,8,9,10-tetrahydro-5H-pyrimido[1,2-a][1,8]naphthyridine-3-carbonitrile N,N-dimethylformamide monosolvate were studied. The compound crystallizes in Triclinic P-1space group with unit cell parameters at 296(2) K as follows: a = 8.6179(4) Å, b =10.3807(5) Å, c =13.0700(5) Å, α =68.369(2)°, β= 75.273(3)°and γ = 77.969(3)°.Crystal data were collected using BRUKER SMART APEX II CCD X-ray diffractometer. The structure was solved by direct methods and refined on F2 by full-matrix least-squares procedures to the final R1 of 0.087using SHELXL programs. The molecular structure is characterized by an intramolecular N-H…O hydrogen bond, which generates an S(6) ring motif. In the crystal, molecules are linked via N-H…O hydrogen bonds, forming infinite bands lying parallel to (110) and composed of alternate R22(12) graph-set ring motifs. These naphthyridine compounds play a significant role in binding with the Influenza virus enzyme.