Hydroxyapatite (HAp) is a well known biomaterial applied in orthopaedic and dental application as bone implants. Bone can easily be infected by microorganisms. In the present study, HAp nanoparticles are capped with Kedrostis foetidissima leaf extract (KHAp) by wet chemical assisted microwave irradiation (method) using calcium hydroxide as calcium source and orthophosphoric acid as phosphorous source. The synthesized samples were characterised by XRD, FT-IR, UV, SEM, EDAX and PL. Further in-vitro analysis was performed using gram positive bacteria Staphyllococcus aureus and Bacillus subtilis and Gram negative Pseudomonas aeruginosa and Escherichia coli, candida albicans fungi. The results of X-ray diffraction (XRD) analysis show the crystalline size of 20.04 nm for pure HAp and 16.71 nm for KHAp. FT-IR results confirm the presence of hydroxyl (O-H) and phosphate (PO43-) groups. The results of UV and PL analysis reveal the band gap energy of 4.06eV. The emission band of KHAp is at 385.91nm. SEM and EDAX predict the spherical shaped morphology with proper proportion of 1.65. In-vitro analysis reveals the significant zone of inhibition for both bacteria and fungi.