Justicia Adhatoda is one of the medicinal plant native to Asia that is widely used in antidiabetic, anti inflammatory, antibacterial properties, treatment of cough and other respiratory ailments. In the present study, few steps procedure method was used to extract the major compound in the leaves.From the leaf extracts, phytochemical tests, measurement of antioxidant activity andantibacterial activities were studied. The results showed that the ethanol leaf extracts contains Phenols, Flavonoids, Tannis, Alkaloids and Saponins. The antioxidant activitywas evaluated in-vitro by radical scavenging DPPH, FRAP and H2O2 method and the disc diffusion method was employed for the antibacterial study on Pseudomonas, Staphylococcus, Bacillus, E.coli using chloramphinicol antibiotic as standard. Antibacterial screening showed that the maximum zone of inhibition for ethanolic extract against E.Coli is around 1.35 cm.
