The efficiency of physical and chemical properties on Extra-long stable Egyptian cotton fiber Giza 96 at different treatment processes with two concentrations of morpholine comparing with conventional treatment with sodium hydroxide (NaOH) was investigated. The sample was pretreated with ferrous sulfate before dyeing with direct dye or natural dye. Physical properties as tensile strength (g/tex), elongation %, fiber length (UHM, and U.I), micronaire reading, reflectance (Rd %), and yellowness (+b) as well as chemical properties as crystallinity degree, moisture regain, accessibility, color strength (K/S), color components and color difference (âˆ†E) was also investigated. The results show that the chemical modified cotton fiber with sodium hydroxide (NaOH) were increased amorphous cellulose %, moisture regain %, accessibility %, fiber uniformity index (U.I) %, elongation %, and micronaire reading compared with morpholine treatments. On the other hand, tensile strength, and fiber length (U.H.M), decreased with a little degree values in morpholine treatments comparing with sodium hydroxide. Improvement in color strength (K/S), and color components was observed using morpholine instead of NaOH. These results were important for finishing textile production and textile industry.