Green nanotechnology has become more popular in recent years to produce metallic nanoparticles since it is inexpensive, requires few preparatory processes, and is sustainable. The current work used Cassia auriculata L. leaf extract to effectively green-synthesize copper nanoparticles (CuO NPs). The generated CuO nanoparticles were properly characterized through UV-Vis, FT-IR, FE-SEM, and EDX analyses. The absorbance peak value at 270 nm in the UV-Vis examination verified that the production of CuO NPs was successful. The FT-IR spectra, which showed characteristic vibrational modes, verified the presence of functional groups on the nanoparticle surface. FE-SEM and EDX validate the spherical structure, and the presence of copper (22.61%) and oxygen (36.35%) was verified by EDX analysis. On DPPH and ABTS assays, CA-CuO NPs showed an exceptional ability to scavenge free radicals. The well diffusion technique was used to assess these NPs' antibacterial activity against clinical isolates, such as S. aureus and E. coli. In conclusion, the green synthesis of CuO NPs using cassia auriculata L. extract is a promising approach for the development of materials with efficient antioxidant and antibacterial properties.
