The present study aims to assess antioxidant and thrombolytic efficacy of Curcuma amada Roxb. (Mango ginger) rhizome extracts terpenoid and phenolic compounds as new therapeutic prospects to serve as fibrinolytic agents. Curcuma amada extract was fractioned with different solvents based on polarity by using pure methanol, ethanol, hydro ethanol, and water. Preliminary phytochemical analysis of all four Curcuma amada extracts revealed that hydroethanolic extract rich in phenol and terpenoid phytocompounds, so hydroethanolic extract alone took for further analysis. Quantitative phytochemical analysis of hydroethanolic extract of Curcuma amada reveals that rich in alkaloid (58±1.08 mg AE/g) content than flavonoid and protein (44±0.78 QE mg/g and 37±0.72 mg/g) contents respectively. Further in vitro thrombolytic analysis reveals hydroethanolic extract of Curcuma amada exhibited the highest and most significant clot lysis (42.35%) of human blood compared to standard streptokinase (29.47%). Hydro ethanolic extract of Curcuma amada rhizome showed seven major compounds peaks in GCMS analysis such as geraniol, curcumin, camphene, eucalyptol, carvacrol, turmerone, nortrachelogenin belongs to terpenoid, phenolic and lignan group of phytocompounds. Further, In silico molecular docking analysis of seven identified compounds from rhizome shown compounds curcumin (-7.8, -7.8 kcal/mol) and nortrachelogenin (-7.1, -6.9 kcal/mol) excellent binding affinity with thrombolytic target proteins tissue plasminogen activator (PDB 1A5H) and coagulation factor XA (PDB 1NFY). Finally molecular dynamic simulation studies have shown curcumin possess an excellent simulation trajectory with tissue plasminogen activator (PDB 1A5H) and coagulation factor XA target protein than with standard drug colchicine. Further clinical study is needed to understand the exact thrombolytic mechanism of Curcuma amada rhizome.
