This work aims to develop an antimicrobial and UV-protective cotton fabric from long staple Egyptian cotton Giza 94 based on forming a coating of nano TiO2-rutile before and after the dyeing process using reactive Remazol yellow 3RS, C.I 167 dye. Pad-dry and microwave curing treatment was used for nano TiO2-rutile fixation on the fabric surface. A new band corresponding to C=O stretching at 1748 cm-1 for treated cotton textile was shown using Infrared analysis. The effect of the coated treated fabric showed a clear microbial resistance against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) and improved the ultraviolet protection factor (UPF). Other properties of the treated fabric, such as tensile strength, elongation at break, color measurements (K/S, Hue, and Chroma), and fastness properties (Washing, light, and perspiration) were measured, and the results showed enhancement compared with the untreated samples.