Industrial activities are the major factor causing the water pollution and degradation of the environment
around us. Sugar industry play important role in economic development of country but the effluent
releases from the industry posses water pollution and causes health hazards. In this study, Pseudomonas fluorescens
strain MK5 was isolated from sugar mill effluent collected from Namakkal district, Tamil Nadu, India
and identified in the basis of morphological, cultural, biochemical characteristics and 16S rRNA analysis.
Free and immobilized cells of Pseudomonas fluorescens strain MK5 were employed for bioremediation of
sugar mill and their Physico-chemical variance were analyzed. Results showed the effluent colour was
changed from dark black to light black in remediated sample. Better results observed in effluent sample remediated
with immobilized cells rather than free cells. Further studies required to understand the principle
mechanism and behavior of this strain in bioremediation process.