The present study reports the synthesis and characterization of ZnO nanoparticles by green synthesis method. The capping agent of Ocimum tnuiflorum leaf extract was used as a reducing agent in the preparation of ZnO nanoparticles. The synthesized ZnO nanoparticles were characterized by X-Ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FT-IR), scanning electron microscope (SEM) and Energy Dispersive Analysis of X−rays (EDAX). The obtained results reveal that the crystalline size, morphology and composition match well with the standard values and will be useful for antibacterial applications.