The present work focuses on the green synthesis of Cerium Oxide nanoparticles using aqueous solution of Coriandrum sativum leaf extract. Cerium nitrate is used as precursor for the preparation and Coriandrum sativum leaf extract is used as an effective reducing agent. The optical properties of biosynthesized CeO2 nanoparticles are characterized by UV-Vis spectroscopy which shows the surface plasmon resonance at 276 nm. The Fourier transform infrared spectroscopy (FT-IR) is used to identify the functional groups of bio-molecules present in the samples. The results of X-ray diffraction (XRD) study revealed that most of the nanoparticles are cubic fluorite structure and grains are crystalline in nature. The study of SEM images shows that as-prepared CeO2 nanoparticles are spherical and fluorite in shape with slight agglomeration and EDAX spectroscopy confirms the presence of Ce and O in the samples with high purity. The antibacterial activity was studied with gram-negative and gram-positive bacteria such as Bacillus subtilis, Bacillus cereus, Klebsiella pneumoniae, Pseudomonas aeruginosa. The gram-positive Bacillus subtilis shows the minimum zone of inhibition when compared with gram-negative.