The adsorption of dye extract from Peristophe roxburghiana (PR) onto cotton was studied to evaluate the mechanism and kinetics of the adsorption process. The study was done at temperatures (50°C, 60°C, and 70°C) and concentrations (2, 2.5, 3, 3.5, 4, 4.5, 5g/ml). Fourier-transform infrared spectroscopy was used to analyze the elemental composition of the dye and UV-VIS spectral revealed that Peristophe roxburghiana (PR) has a maximum wavelength of 580nm and the functional group showed a semblance of an anthocyanin dye. The adsorption mechanism of dye extract from Peristophe roxburghiana leaves onto cotton fabric was greatly influenced by temperature, time, and concentration each parameter influenced the molecular mobility of the dye as it fixed onto the cotton. Its adsorption attributes revealed that apart from surface physisorption exiting between the dye and cotton, there was ionic interaction occurring between the dye and the cotton sample is given chemisorption, with its adsorption kinetics obeying pseudo-second-order adsorption model perfectly.