Ethanol extract of Prosopis Juliflora was investigated as an inhibitor for mild steel corrosion in 1M HCl using different techniques like weight loss, potentiodynamic polarization, and electrochemical impedance spectroscopy (EIS). Weight loss method implies that efficiency of inhibitor increases with increase of concentration and temperature. Polarization measurement reveals that Prosopis Juliflora acts as a mixed type inhibitor for mild steel in 1M HCl. The results of Impedance spectroscopy indicates that the value of Rct increases with increase in the concentration of inhibitor. The surface morphology of Prosopis Juliflora was analysed by FT-IR and SEM methods. The antimicrobial activity of Prosopis Juliflora was also investigated in order to confirm the zone of inhibition.