In pursuing sustainable bio-economy and bio-refinery advancements, enzymatic hydrolysis plays a pivotal role in converting starch-based feedstock into valuable products. Amylase, an enzyme crucial for starch hydrolysis, faces challenges in stability and reusability in industrial applications. This study explores the Immobilization of amylase on alginate to enhance enzyme stability and reusability while evaluating its hydrolysis efficiency. Results indicate immobilized amylase's improved thermal and pH stability, particularly at elevated temperatures and optimal pH levels. However, the immobilized enzyme demonstrated reduced hydrolysis efficiency compared to its free form, likely due to diffusion limitations and restricted enzyme mobility. The findings suggest that while Immobilization offers practical advantages, further optimization is needed to enhance enzyme efficiency for broader industrial applications.
