The most cutting-edge field of the twenty-first century is nanotechnology. In comparison to their bulk counterparts, nanoparticles have grown significantly in importance due to their special features. Zinc oxide nanoparticles are among the most significant metal nanoparticles because they are used in optical, physical, and antibacterial characteristics, gas sensors, biosensors, cosmetics, medication delivery systems, and other products. ZnO NPs can be created via a variety of chemical processes, including the hydrothermal process, vapor transfer method, and precipitation method. Due to its great biocompatibility, affordability, and low toxicity, ZnO NPs have grown to become one of the most widely used metal oxide nanoparticles in biological applications over the past 20 years. By adjusting the growth temperature and precursor concentration, the hydrothermal technique was used to create stable, OH free zinc oxide (ZnO) nanoparticles. Fourier Transform Infrared Spectroscopy (FT-IR) and X-ray diffraction (XRD) results supported the formation of ZnO nanoparticles. It has been determined that the typical particle size is between 60 and 80 nm by particle size measurement using zeta sizer. By creating an agar-agar culture plate, ZnO nanoparticles as adsorbent materials were evaluated for the antimicrobial test by taking different microbial species. The zone of inhibition study was proved that ZnO nanoparticles were successful in limiting the bacterial growth in the petriplate culture media. According to experimental findings, among the chosen materials, ZnO NPs have demonstrated the best antifungal activity.